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Stimulation of taxane
production in suspension cultures of Taxus yunnanensis
by oligogalacturonides
Yu Ting Guo and
Jian Wen Wang*
School of
Pharmaceutical Sciences, Soochow University, Suzhou 215006,
P.R. China.
*Corresponding author. E-mail:
jwwang@suda.edu.cn.
Tel: 86-512-67410163. Fax: 86-512-6588-0031.
Accepted
3 June, 2008 |
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When Taxus yunnanensis cells of 12-day-old cultures were
exposed to oligogalacturonide (OGA) at 20-80 μg ml-1
for 3 days, it was found that paclitaxel
and 10-deacetyl baccatin III (10-DAB III) content peaked at
23 and 24 μg g-1 dry wt, respectively. OGA
treatment increased taxane release percentage (extracellular
release ratio) from 10 to 90%. The maximum production of
paclitaxel and 10-DAB III in cultures elicited with OGA at
40 μg ml-1 was demonstrated to be 0.5 and 1.0 mg
l−1, a 3.6 and 3.4-fold that of the control. This
is the first report on the stimulation of taxoid production
by OGA.
Key
words:
10-deacetyl baccatin III production, oligogalacturonides,
paclitaxel producton, Taxus yunnanensis. |
