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Free radical scavenging
activity of Pterogyne nitens Tul. (Fabaceae)
Luis Octávio Regasini1*,
Camila Martins de Oliveira1, José Carlos Rebuglio
Vellosa2, Olga Maria Mascarenhas de Faria
Oliveira2, Dulce Helena Siqueira Silva1
and Vanderlan da Silva Bolzani1
1Department
of Organic Chemistry, Institute of Chemistry, São Paulo
State University, Araraquara, Brazil.
2Department
of Biochemistry and Technological Chemistry, Institute of
Chemistry, São Paulo State University, Araraquara, Brazil.
*Corresponding author.
E-mail:
regasini@iq.unesp.br. Tel: +55-016-3301-6660. Fax:
+55-016-3322-7932.
Accepted
30 October, 2008 |
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As part of our ongoing research on antioxidant agents from
Brazilian flora, twenty extracts and fractions obtained from
Pterogyne nitens Tulasne (Fabaceae) were screened for
free radical scavenging activity by using ABTS
[2,2’-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)]
and DPPH (2,2-diphenyl-1-picrylhydrazyl-hydrate) radicals
colorimetric assay and
β-carotene
bleaching test.
The
strongest activity was found in ethyl acetate fraction from
the stem barks, exhibiting IC50 values (in µg/ml)
of 2.10 ± 0.1 and 10.2 ± 0.3 on ABTS•+ and DPPH•,
respectively. Additionally, chromatographic fractionation of
stem barks yielding myricetin, quercitrin and mirycetrin,
three flavonols with remarkable antioxidant activity.
Key
words:
Antioxidant, free radical scavenging activity, DPPH, ABTS,
Pterogyne nitens, Leguminosae, Fabaceae,
Caesalpinioideae, flavonoid, flavonol. |
