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Determination of
antimicrobial activity and production of some metabolites by
Pseudomonas aeruginosa B1 and B2 in sugar beet
molasses
Dilsad Onbasli1*
and Belma Aslim2
1Kastamonu
University,
Faculty
of Science and Arts, Department of Biology, 37100,
Kastamonu-Turkey.
2Gazi
University, Faculty of Science and Arts, Department of
Biology, 06500, Teknikokullar, Ankara-Turkey.
*Corresponding author. E-mail:
donbasili@kastamonu.edu.tr .
Tel: +0903662154924. Fax: +903662154969.
Accepted
28 October, 2008 |
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In the
present study, exopolysaccharide (EPS), pyocyanin,
rhamnolipid productions, total cell proteins and
antimicrobial activities were investigated in two strains of
Pseudomonas aeruginosa (B1 and B2). Exopolysaccharide,
pyocyanin and rhamnolipid production of strains were
determined in nutrient broth medium (NB) as control and
different sugar beet molasses concentrations (1-5 % w/v) at
different periods (24, 48 and 72 h) by a spectrophotometric
method. The exopolysaccharide, pyocyanin and rhamnolipid
productions of both strains increased after incubation when
5% (w/v) of molasses were used. Therefore the results
disclose the correlations between the increasing molasses
concentrations and EPS, pyocyanin and rhamnolipid
productions of P. aeruginosa B1 and B2 strains (P≤0.01).
Bacillus
subtilis
ATCC 6633 was more sensitive than
Escherichia
coli
11230 to the inhibitory effect of P. aeruginosa B1
and B2. Moreover, these strains exhibited the highest
antimicrobial activity against both test bacteria in sugar
beet molasses (5% w/v) at 72 h. Significant increases in
total cell protein and antimicrobial activity of P.
aeruginosa B1 and B2 were observed with increasing the
molasses concentrations (P≤0.01). The results also
indicated that strains having high total cell protein were
higher antimicrobial activity, EPS, pyocyanin and
rhamnolipid productions.
Key
words: Pseudomonas aeruginosa, exopolysaccharide,
pyocyanin, rhamnolipid, antimicrobial activity, sugar beet
molasses |
