|
Identification of sugarcane interspecies hybrids with RAPDs
H. Y. Zhang1,2, F. S. Li1,3*,
l. L. He3, H. Q. Zhong3, Q. H. Yang3
and S. C. He3
1Key
Laboratory of Agricultural Biodiversity for Pest management,
Ministry of Education, Yunnan Agricultural University,
Kunming, Yunnan Province, People’s Republic of China,
650201.
2Biotechnology
laboratory; Southwest Forestry College; White Dragon Temple;
Kunming, Yunnan Province-650224; People’s Republic of China.
3College
of Agronomy and Biotechnology of Yunnan Agricultural
University, Kunming, Yunnan Province, People’s Republic of
China, 650201.
*Corresponding author. E-mail:
lfs810@sina.com. Tel:
81-0871-5227726. Fax: 81-0871-5227704.
Accepted 22 February, 2008 |
|
Identification of “Saccharum officinarum ×
Erianthus fulvus” F1 hybrids was performed
with random amplified polymorphic DNA (RAPD) analysis. Of
280 RAPD primers used, two primers, OPA-19 and OPN-11, were
found to be the most suitable for identification of the
hybrids. And the hybrids facticity-check-out rate was 70.6
and 68.3%, respectively.
Key words:
Sugarcane hybrids, identification, RAPD. |
