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Determination
of allele frequencies in nine short tandem repeat loci of
five human sub-populations in Botswana
Mpoloka S.W.1, Kgotlele T.1
and Wally A.2.
1Department
of Biological Sciences, University of Botswana, P/Bag 00704,
Gaborone, Botswana.
2Botswana
Police Forensic Science Laboratory P/Bag 0012, Gaborone,
Botswana.
*Corresponding author. E-mail:
mpoloka@mopipi.ub.bw.
Accepted 15 February, 2008 |
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Allele frequencies for nine short tandem repeat (STR) loci
from the AmpFlSTR® Profiler Plusä
PCR Amplification Kit
were determined in five subpopulations across Botswana and
tested for suitability for use in individual
identification. DNA was extracted from whole blood samples
collected from 150 unrelated individuals. Targeted regions
of DNA (vWA, FGA, D3S1358, D5S818, D7S820, D8S1179, D13S317,
D18S51, D21S11 and the sex determining locus Amelogenin)
were amplified using multiplexed PCR. The alleles showed a
high degree of polymorphism, with heterozygosity above 67%,
indicating that there is no significant inbreeding within
the subpopulations. Matching probability was below 0.2 hence
power of discrimination was high, indicating that the
alleles from the five sub-populations can be used in human
identifications. The present study is the first reported
attempt at determining allele frequencies of subpopulations
in Botswana and could possibly be used in developing a
national DNA database.
Key words:
DNA typing, short tandem repeat (STR), polymerase chain
reaction, allele frequency, Botswana. |
