Full Length Research Paper

Biological hardening and genetic fidelity testing of micro-cloned progeny of Chlorophytum borivilianum Sant. et Fernand.

Archana Mathur, Ajay Kumar Mathur*, Priyanka Verma, Shrawan Yadav, Moti Lal Gupta and Mahendra P. Darokar

Central Institute of Medicinal and Aromatic Plants (CIMAP) PO CIMAP; Picnic Spot Road; Lucknow –226015 India.

*Corresponding author. E-mail: akmcath@yahoo.com or ak.mathur@cimap.res.in. Tel: ++91 522-2717557, 2359632 (Office); ++91 -0-9415419061 (mobile). Fax: ++91 522-234666. CIMAP Publication No.2007-60J.

Accepted 25 January, 2008

The micro-cloned plantlets of Chlorophytum borivilianum registered more than 95% establishment in soil following treatment with various bio-inoculants namely; Glomus aggregatum, Trichoderma harazianum and Piriformospora indica whereas Azospirullum sp. (CIM-azo) and Actinomycetes sp. (CIM-actin) showed only up to 85% plantlet establishment. The un-rooted shoots were also treated with these bio-inoculants, for in vivo root induction and increased survival rate/establishment frequency when transferred to soil. The un-rooted shoots also showed in vivo rooting (50%) when treated with mycorrhiza Glomus aggregatum (VAM) and Trichoderma harazianum. The genetic fidelity testing of micro-cloned, bio-hardened progeny based on a RAPD analysis using 40 random decamer DNA primers indicated a strong uniformity in relation to the parent genotype.

Key words: Chlorophytum borivilianum, bio-inoculants, bio-hardening, genetic fidelity, RAPD analysis.