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Analysis of
in vivo
binding of yeast heat shock factor to promoter DNA
K.H. Liu1,2*,
L.M. Zhang2*, X.W. Ding1,
B.W. Deng1,
and W.Q. Chen1
1Bio-resources
Key Laboratory of Shaanxi, Shaanxi University of Technology,
Shaanxi Hanzhong 723001, P. R. China.
2Key
Laboratory for Conservation and Utilization of Bio-resources,Yunnan
University, Kunming, 650091, P. R. China.
*Corresponding author. E-mail:
kaihhui168@yahoo.com.cn;
zhanglm@ynu.edu.cn
Accepted
13 February, 2008 |
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The in
vivo binding of yeast heat shock factor (HSF) to
promoter domains of stress-inducible genes was examined
using chromatin immunoprecipitation (ChIP) combined with PCR.
The promoter-specfic PCR amplification of DNA
immunoprecipitated with antibodies against HSF showed that
yeast HSF was bound constitutively to the promoters of
GTT2, YLL058W, COQ6, GND2 and
YGR066C genes, induciblely to the MRS2 gene. HSF-binding
consensus sites prediction indicated the promoter sequences
of the stress-inducible genes contain heat shock elements (HSEs).
These results suggest the genes are potential HSF-regulated
targets.
Key
words:
Yeast, heat shock factor, heat shock elements, chromatin
immunoprecipitation. |
