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Biological potantial of
some Iranian Trichoderma isolates in the control of
soil borne plant pathogenic fungi
Behzad Hajieghrari1*,
Mousa Torabi-Giglou1, Mohammad Reza Mohammadi2
and Mahdi Davari3.
1Department
of Plant Production, Moghan Junior College of Agriculture,
University of Mohaghegh – Ardabili, Ardabil, Iran.
2Department
of Plant Protection, Faculty of Agriculture, Islamic Azad
University Branch, Varamin, Iran.
3Department
of Plant Protection, Faculty of Agriculture, University of
Mohaghegh Ardabili, Ardabil, Iran.
*Corresponding author. E-mail:
bhajieghrari@uma.ac.ir. Tel: +989143186861.Fax:
+984527463417.
Accepted
20 March, 2008 |
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In this
study the in vitro potential of six selected Iranian
isolates of three species of Trichoderma (Trichoderma
hamatum T614, T. hamatum T612, Trichoderma
harzianum T447, T. harzianum T969, Trichoderma
virens T523 and Trichoderma sp. T) were evaluated
against five isolates of soil borne phytopathogenic fungi (Fusarium
graminearum, Rhizoctonia solani (AG4 and AG5),
Macrophomina phaseoli and Phytophtora cacturum)
in dual culture techniques and through production of
volatile and non-volatile inhibitors, and the pH and
temperature effects on Trichoderma mycelial growth
were also evaluated. All Trichoderma isolates had a
marked statistical inhibitory effect on mycelial growth of
the pathogens in dual culture compared with controls.
Maximum inhibitions occurred in F. graminearum-T.
hamatum T614
interaction. Significant pathogen colony growth inhibitions
were observed when exposed to the trapped atmosphere from
culture of the Trichoderma. F. graminearum was
most susceptible to the volatile inhibitors produced by
T. hamatum T612 (%inhibition = 48.65). Medium filtrate
obtained the Trichoderma isolate culture also were
effected on the pathogen species significantly. Maximum
growth inhibition was observed in radial growth of F.
graminearum by T. hamatum T612 non volatile
metabolites (%inhibition = 38.3).
Evaluation of pH and temperature effects on
Trichoderma isolates mycelial growth
showed that Trichoderma strains were found to be able
to display activities under a wider range of pH values.
Also, Trichoderma strains are mesophilic.
Key
words:
Trichoderma spp., biological potantial, soil borne
phytopathogenic fungi, Iran. |
