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Lectins
binding during alloxan-induced diabetes in rat soleus muscle
Nursel Gül*, Suna Cebesoy and Nesrin
Özsoy
Ankara University, Faculty of Science, Deparment of Biology,
06100 Tandoğan, Ankara, Turkey.
*Corresponding author. E-mail:
ngul@science.ankara.edu.tr. Tel: 90-(312)-212 67 20 ext
1057. Fax: 90-(312)-2232395.
Accepted 5 March, 2008 |
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Membrane structural changes of soleus muscle of alloxan-diabetic
rats were detected with a panel of six biotinylated lectins.
Samples of muscles were obtained from normal and diabetic
rats. The biotinylated lectins in staining were detected by
avidin-peroxidase complex. Lectin stainning of soleus muscle
cryostat sections from alloxan-diabetic rats were compared
with similar cryostat sections from normal rats. All lectins
were bounded to soleus muscle cell membranes of normal and
diabetic rats except the Ulex europeus agglutinin I (UEA-I).
UEA-I was not stained for cell membranes of soleus muscles
of normal and diabetic rats. But intercellular areas of
normal and diabetic soleus muscle were stained for UEA-I.
Griffonia simplicifolia I (GS-I) lectin was bounded more
strongly to cell membrane of diabetic rats than other
lectins. Not only cell membranes but also cytoplasmic
miyofibrills of diabetic muscle cells were stained by
Griffonia simplicifolia (GS1) lectin. It was concluded
that cell membranes of soleus muscle of diabetic rats were
differently stained for lectins than those of normal rats.
Our data can supplement on the use
of lectins as probes for changes in carbohydrate-containing
constituents of the alloxan-diabetic muscle cell membrane
information obtained from normal and diabetic
Key words:
Diabetes, alloxan, Wistar rat, soleus muscle, lectin
staining. |
