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Screening and assessment
of laccase producing fungi isolated from different
environmental samples
Buddolla Viswanath, M. Subhosh Chandra, H. Pallavi and B.
Rajasekhar Reddy*
Department
of Microbiology, Sri Krishnadevaraya University, Anantapur -
515 003. A.P. INDIA.
*Corresponding author. E-mail:
buddolla@yahoo.co.in.
Accepted 8
March, 2006 |
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Laccase is a copper-containing polyphenol oxidase that acts
on a wide range of substrates. This enzyme is found in many
plant species and is widely distributed in fungi including
wood-rotting fungi where it is often associated with lignin
peroxidase, manganese dependent peroxidase, or both. Because
of its importance in bioremediation, fungal cultures were
screened for laccase positive production by plate test
method using the indicator compound guaiacol. Out of 12
cultures tested, six cultures were found to be laccase-positive
with Stereum ostrea and Phanerochaete
chrysosporium being the best potential cultures.
Laccase production on 5 different liquid media was compared
using these two white rot fungi.
Key words: Laccase, guaiacol, isolation, Stereum ostrea,
Phanerochaete chrysosporium. |
