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TDZ x
IBA induced shoot regeneration from cotyledonary
leaves and in vitro multiplication in
safflower (Carthamus tinctorius L.)
Dilek Başalma1, Serkan Uranbey2*,
Semra Mirici3 and Özer Kolsarici1
1Department
of Field Crops, Faculty of Agriculture, University of
Ankara, 06110, Dışkapı, Ankara, Turkey.
2Central
Research Institute for Field Crops, Yenimahalle, Ankara,
Turkey.
3Faculty
of Education, University of Akdeniz, Antalya, Turkey.
*Corresponding author. E-mail:
basalma@agri.ankara.edu.tr.
Abbreviations:
TDZ, thidiazuron; IBA, indole-3-butyric acid; MS, Murashige
and Skoog medium; BAP, N6-benzylamino-purine; and
NAA, α-naphthaleneacetic acid.
Accepted 22 January, 2008 |
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A high frequency adventitious shoot regeneration protocol
for safflower (Carthamus tinctorius L.) cv. Dinçer
with high yield (Turkish cultivar) using cotyledonary leaves
of 10 day-old seedlings were optimized by studying the
influence of different combinations of thidiazuron (TDZ)
and indole-3-butyric acid (IBA). Cotyledonary leaves were
cultured on Murashige and Skoog medium (MS) supplemented
with different concentrations and combinations of TDZ and
IBA. The highest percentage of regenerated shoots (33.33%)
and the highest number of shoots per explant (6.5) occurred
on a MS medium containing 0.5 mg/l TDZ and 0.25
mg/l IBA. Furthermore, cotyledonary nodes and
meristem tips of 14 day-old seedlings were cultured on
Murashige and Skoog (MS) medium supplemented with various
concentrations of N6-benzylamino-purine (BAP)
alone or combination of α-naphthalene acetic acid (NAA).
Direct multiple shoots from cotyledonary nodes and meristem
tips developed within 18 - 21 days in most media tested.
100% shoot multiplication was achieved from cotyledonary
node and meristem tip on a range of MS media supplemented
with different concentrations of BAP and NAA.
Key words:
Carthamus tinctorius, regeneration,
cotyledonary leaves. |
