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Analysis of genetic
variability among plantain cultivars (Musa paradisiaca L.)
using arbitrarily primed PCR technique
Agoreyo, B. O.1,
Golden, K. D.2 and Brown, S. E.2
1Department
of Biochemistry, Faculty of Life Sciences, University of
Benin, Benin City, Nigeria.
2Department
of Basic Medical Sciences, Biochemistry Section and
Biotechnology centre, Faculty of Medical Sciences,
University of the West Indies, Mona, Jamaica.
*Corresponding author. E-mail:
agoreyobo@yahoo.com.
Accepted
5 March, 2008 |
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The genetic
variability among 6 cultivars of plantain (Musa
paradisiaca L.) grown in Jamaica and Nigeria was
studied, using arbitrarily primed PCR (AP-PCR) technique.
The cultivars included Maiden plantains and Horse plantain
grown in Jamaica: Bini, Ayo and Igbiya plantains grown in
Nigeria. DNA fragment band positions were obtained with
fragment sizes ranging from 0.438 to 1.926 kb. The single
distance matrix calculations and the generated dendrogram
revealed a clustering together of plantain cultivars across
sources of propagation. The analysis showed that the
plantain cultivars studied, were split into two clusters,
One group consisted of Maiden plantains and Horse plantain
from Jamaica and the second contained Bini, Ayo and Igbiya
of Nigeria. Cultivars Ayo and Igbiya were closest while
Horse and igbiya were one of the farthest apart, in genetic
relatedness.
Key
words:
Musa paradisiaca, plantain, AP-PCR analysis, genetic
diversity. |
