|
Free radical scavenging
activity and phenolic content of Cassia sophera L.
Atiqur Rahman1, 2 *, M. Mizanur
Rahman3, 4, Md. Mominul Islam Sheik3,
M. Mashiar Rahman3, Shabah Mohammad
Shadli3 and M. F. Alam5
1Department
of Biotechnology, Daegu University, Kyoungsan 712-714,
Korea.
2Department
of Applied Chemistry and Chemical Technology, Islamic
University, Kushtia 7003, Bangladesh.
3Department
of Biotechnology and Genetic Engineering, Islamic
University, Kushtia 7003, Bangladesh.
4Dipartimento
di Biotecnologie Agrarie, Viale
dell’ Università 16, 35020 - Legnaro (Padova), Italy.
5Department
of Botany, Rajshahi University, Rajshahi, Bangladesh.
*Corresponding author. E-mail:
marahman12@yahoo.com.
Tel.: +82-53-850-4416; Fax: +82-53-850-6559
Accepted 31 March, 2008 |
|
Dried methanol extract of
leaves of Cassia sophera L. was dissolved in
distilled water, and then fractioned by re-extracting with
n-hexane, chloroform, and ethyl acetate, subsequently. The
free radical scavenging activity (FRSA) of methanol extract
and various fractions of methanol extract was determined by
using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The FRSA
of ethyl acetate fraction was superior to all other
fractions (IC50 = 15.42 μg/ml), which was higher
than synthetic antioxidant butylated hydroxyanisole, BHA
(18.25 μg/ml). The amount of total phenolic compounds was
further determined. The total phenolic content in EtOAc
fraction (13.25%) was the highest as compared to other
extracts. The results of this study suggest that this plant
could serve as a source of natural antioxidants and
preservative agents with potential applications in food
industries.
Key words:
Cassia sophera L., radical scavenging activity,
phenolic content. |
