African Journal of Biotechnology Vol. 6 (15), pp. 1840-1847, 6 August 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Fatty acid production by four strains of Mucor hiemalis grown in plant oil and soluble carbohydrates

Sâmia M. Tauk-Tornisielo*, Joelma Maurício Vieira, Maria Cecília, V. S. Carneiro and José Silvio Govone

Centro de Estudos Ambientais, Universidade Estadual Paulista (UNESP), Bairro Bela Vista, Rio Claro-13.506-900, SP, Brazil.

*Corresponding author. E-mail: seb@rc.unesp.br.

Accepted 2 July, 2007

Four Mucor hiemalis strains (M1, M2, M3 and M4), isolated from soil at a depth of 0 - 15 cm in the Juréia-Itatins Ecology Station (JIES), in the state of São Paulo, Brazil and were evaluated for the production of γ-linolenic (GLA) and other unsaturated fatty acids. Five growth variables (temperature, pH, carbon source, nitrogen source, and vegetable oils) were studied. Liquid media containing 2% vegetable oil (palm oil, canola oil, soybean oil, sesame oil, or sunflower oil) or 2% carbohydrate (fructose, galactose, glycerol, glucose, lactose, maltose, sucrose, sorbitol or xylose) and 1% yeast extract as a nitrogen source were used. The greatest biomass production was observed with M3 and M4 strains in palm oil (91.5 g l^-1) and sunflower oil (68.3 g l^-1) media, respectively. Strain M4 produced greater quantities of polyunsaturated acids in medium containing glucose. The GLA production in the M4 biomass was 1,132.2 mg l^-1 in glucose medium. Plant oils were inhibitors of fatty acid production by these strains.

Key words: Fatty acid production, Mucor hiemalis, polyunsaturated acids, γ-linolenic acid..