African Journal of Biotechnology Vol. 6 (15), pp. 1817-1820, 6 August 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Hairy root induction from hypocotyl segments of groundnut (Arachis hypogaea L.)

A. KARTHIKEYAN, S. PALANIVEL. S. PARVATHY and R. BHAKYA RAJ

Department of Microbiology and Biotechnology, JJ College of Arts and Science,

Pudukkottai – 622 404, India.

*Corresponding author. E-mail: mdukarthimicro@yahoo.com.

Accepted 20 June, 2007

Hairy roots were induced from hypocotyl explants excised from seven day old aseptically grown seedlings of groundnut using Agrobacterium rhizogenes 15834. The percentage of hairy root induction and number of hairy roots per ex-plant varied with infection period.  The suitable co-cultivation period was 48 h. The hairy roots were fast growing, thin, slender and sometimes having branches which varied in their morphological nature. The cefotaxime concentration of 250 mgL^-1 was found to be most suitable for hairy root induction in groundnut.

Key words: Arachis hypogaea; Agrobacterium rhizogenes, hairy root induction.