African Journal of Biotechnology Vol. 6 (17), pp. 1987-1989, 5 September 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Short Communication

Analysis of genetic variation in different banana (Musa species) variety using random amplified polymorphic DNAs (RAPDs)

Pankaj Kumar Jain¹, Mohan Lal Saini²*, Hardik Pathak¹ and Virendra Kumar Gupta¹

¹Departmant of Biotechnology, Mahatma Gandhi Institute of Applied Sciences, Jaipur, India.

²Biotechnology Lab, Department of Botany, UOR, Jaipur, India.

*Corresponding author. E-mail: monaphd2005@yahoo.co.in.

Accepted 27 July, 2007

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value as good dietary source. Here, we analyze the genetic relationship of four different banana varieties that are cultivated in south India. Random amplified polymorphic DNAs (RAPDs) fingerprinting of these banana varieties (Grand Naine, Red Banana, Nendran and Rasthali) carried out by three primers (OPA-19, OPB-18, OPD-16) led to DNA amplification. 43.47% of the amplification products were monomorphic (common to all the genotypes), whereas 30.43% were unique, but only 26.08% revealed the relationship between these genotypes.

Key words: DNA fingerprinting, RAPD analysis, Taq DNA polymerase, primers.