African Journal of Biotechnology Vol. 6 (21), pp. 2493-2496, 5 November 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Alkaline Protease from Bacillus firmus 7728

Kiranmayee Rao and M. Lakshmi Narasu*

Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad – 500085, India.

*Corresponding author. E-mail: raorskiran@rediffmail.com. Tel./Fax: +91040 23156129.

Accepted 19 October, 2007

Extracellular alkaline protease producing Bacillus firmus MTCC 7728 was isolated from the soil samples taken from the leather factories in Nacharam industrial area, Hyderabad. Maximum activity was found after 48 h of fermentation. Optimum pH and temperature for maximum enzyme activity were 9 and 40°C, respectively. The potential of mesophilic alkaline protease produced by Bacillus firmus MTCC 7728 in various industries is yet to be exploited. 

Key words: Isolation, Extracellular alkaline protease, Bacillus firmus MTCC 7728.