African Journal of Biotechnology Vol. 6 (23), pp. 2682-2686, 3 December 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Comparative analysis of inter population genetic diversity in Puntius filametosus using restriction fragment length polymorphism (RFLP) analysis

Jeyaraj Antony Johnson¹, Rajayanian Pusphabai Rajesh³, Lidwin Anna Mary³ and Muthukumarasamy Arunachalam²

¹Department of Biology, Eritrean Institute of Technology, Ministry of Education, Asmara, North East Africa.

²Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkruichi – 627 412, Tamil Nadu, India.

³Department of Biotechnology, Malankara Catholic College, Mariagiri, Kaliakavilai – 629 153, Tamil Nadu, India.

*Corresponding author. Email: jajohny@rediffmail.com.

Accepted 12 November, 2007

The genetic variation in different population of the freshwater cyprinid Puntius filamentosus was studied using restriction fragment length polymorphism (RFLP) analysis. Samples were collected from five different locations of southern Western Ghats, India. The morphometric characters of population from Alancholai showed little variation when compared with other population. The genomic size of the different population of P. filamentosus found between 3.45 and 3.80 ng/mg. The result of RFLP analysis showed that the population from Alancholai had distinct fragment length and scored high band volume (12.430 nmoles). The result of cluster analysis showed that Alancholai population had distinct genetic structure and it did not cluster with other population. The study inferred that the population from Alancholai appeared to be unique among the other population of P. filamentosus.

Key words: Puntius filamentosus, genetic diversity, RFLP, Western Ghats, India.