African Journal of Biotechnology Vol. 6 (9), pp. 1132-1135, 2 May 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Isolation and purification of membrane-bound cytochrome c from Proteus mirabilis

Muhammad Atif Zia¹*, Muhammad Shahid Nazir, Tariq Ismail and Muhammad Hassan Qureshi

Biochemistry Laboratory, Department of Chemistry, Government College University, Lahore, Pakistan.

*Corresponding authors E-mail: zea_chemist@yahoo.com.

Accepted 4 May, 2007

In the present studies, respiratory chain pathogenic bacterium, Proteus mirabilis, was investigated. In the first phase, growth profile study was performed to optimize the P. mirabilis growth. Maximum bacterial growth could be obtained between 10 – 12 h of culturing time. Down-stream processing was performed by using sonication, ultracentrifugation and detergent solubilization techniques. Partially purified respiratory contents were analyzed spectrophotometrically. Pyridine-ferrochrome and redox spectra showed the presence of heme-c.

Key words: Cytochrome c, respiratory chain and Proteus mirabilis.