African Journal of Biotechnology Vol. 6 (7), pp. 847-852, 2 April 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

The effect of interaction between Lipoprotein Lipase and ApoVLDL-II genes on fat and serum biochemical levels

Hassan Hussein Musa^1,2, Guo Hong Chen^1* and Bi Chun Li¹

¹College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

²Department of Animal Production, Faculty of Veterinary Science, University of Nyala, Sudan.

*Corresponding author. E- mail: ghchen@yzu.edu.cn. or hassan_hm30@yahoo.com

Body weight, abdominal fat weight and serum biochemical levels were determined from lean and fat chicken breeds at 12 weeks of age. Single nucleotide polymorphism (SNP) in apoVLDL-II and lipoprotein lipase genes was screened by PCR-SSCP and detected by direct sequencing. Lipoprotein lipase gene frequency was found to be significantly different (P < 0.01) in lean chicken whereas it was non-significantly different in fat chicken. SNP in apoVLDL-II and lipoprotein lipase genes significantly (P < 0.05) affected body weight and fat weight. Similarly their interaction significantly (P < 0.05) affected body weight and fat weight. However, no significant difference was observed in the percentage of abdominal fat. SNP in apoVLDL-II and lipoprotein lipase genes significantly (P < 0.05) affected total cholesterol and high density lipoprotein. More likely, the interaction of apoVLDL-II and lipoprotein lipase significantly affect total cholesterol, triglyceride, high density lipoprotein, very low density lipoprotein and low density lipoprotein.

Key words: Interaction, lipoprotein lipase, apoVLDL-II, fat, serum biochemical levels.