African Journal of Biotechnology Vol. 6 (16), pp. 1874-1878, 20 August 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Hormonal regulation for callogenesis and organgenesis of Artemisia absinthium L

Muhammad Zia*, Riaz-ur-Rehman and Muhammad Fayyaz Chaudhary

Plant Physiology Laboratory, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan 45320.

*Corresponding author. E-mail: zia_qau@yahoo.com.

Abbrevations: 2,4-D, 2,4-dichloro phenoxy acetic acid; NAA, nephthalene acetic acid; IBA, indol butyric acid; IAA, indol acetic acid; BAP, benzyl amino purine; Kin, Kinetin.

Accepted 14 June, 2007

Callus cultures were induced from leaf and stem explants of Artemisia absinthium, at different auxin and cytokinin concentrations. Moderate concentrations of growth regulators either in combination or in single in MS medium produced friable, light green and non-embryogenic callus from both explants. These totipotent cells gave rise to shoots when transferred to same or different growth regulator containing medium as second subculture. Complete rooting was achieved on full and half strength basal MS medium supplemented with different auxin concentrations. Synergetic effect of plant growth regulator plays an important role in callus induction and cell differentiation.   

Key words: Artemisia absinthium, callogenesis, organogenesis, plant growth regulators.