African Journal of Biotechnology Vol. 6 (22), pp. 2590-2595, 19 November 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Optimization of growth and extracellular glucoamylase production by Candida famata isolate

Lagzouli Mohamed¹*, Mennane Zakaria¹, Aitounejjar Ali², Wafae Senhaji³, Ouhssine Mohamed¹, Elyachioui Mohamed¹, Berny EL Hassan¹ and Jadal Mohamed

¹Laboratory of Microbial Biotechnology, University IBN TOFAIL, Department of Sciences, BP 133, 14000 Kenitra-Morocco.

²National Institute of Agronomic Researches, INRA, BP: 589 Settat 26000, Morocco.

³Laboratoire de Recherche et d'Analyses Médicales de la Gendarmerie Royale, avenue Ibn Sina-Agdal-Rabat, B.P 8087, 10000, Morocco.

*Corresponding author. E-mail: lagzoulimohamed@yahoo.fr.

Accepted 16 March, 2007

Candida famata was isolated from traditional Moroccan sourdough. It exhibited high glucoamylase and biomass production. Starch induces high glucoamylase production C. famata with maximum glucoamylase activity at 5 g/L. Glucose stimulates good production in biomass but strongly inhibits glucoamylase production. Among the sources of nitrogen tested, yeast extract and the (NH₄)₂HPO₄ gave maximum glucoamylase and biomass after 72 h of incubation in liquid medium at 30°C, pH 5 and 105 rpm.

Key words: Glucoamylase, yeast, optimization, biotechnology, Candida famata.