African Journal of Biotechnology
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African Journal of Biotechnology Vol. 6 (6), pp. 672-676, 19 March 2007 ISSN 1684–5315 © 2007 Academic Journals
Cloning and homologic analysis of Tpn I
gene in silkworm Bombyx mori
Zhao Yuan1, 2, Yao Qin1,
Tang Xudong1, Wang qiang1, Yin huijuan1,
Hu Zhigang1, Lu Jian1 and Chen Keping1* 1Institute
of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, P.
R. China. 2
Sericultural Research Institute, Chinese Academy of Agricultural Sciences,
212018, China.
*Corresponding author. E-mail:
kpchen@ujs.edu.cn,
Tel: 86-511-8791923. Fax:
86-511-8791923.
Accepted 16
February, 2007 |
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| Abstract | |||||
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The troponin complex is composed of three subunits, Troponin C (the calcium sensor component) and Troponin T and I (structural proteins). Tpn C is encoded by multiple genes in insects, while the Tpn T and Tpn I proteins are encoded by single genes. Tpn I binds to actin and Tpn T binds to tropomyosin. We cloned and sequenced the Tpn I (AY873787) gene from Bombyx mori that encodes 225 amino acids and contains a conserved motif seen in Drosophila virilis and Anopheles gambiae. Bioinformatic analysis suggests that its deduced amino sequence shares 81.3 and 78.7% homology with the Tpn I genes of A. gambiae and D. virili, respectively.
Key words: Tpn I, Bombyx mori, troponin, homology. |
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