African Journal of Biotechnology Vol. 6 (6), pp. 707-712, 19 March 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Standardization of growth and fermentation criteria of Lasiodiplodia theobromae for production of jasmonic acid

Pinakin C. Dhandhukia and Vasudev R. Thakkar*

B & R Doshi School of Biosciences, Sardar Patel Maidan, Sardar Patel University, Vadatal Road, Bakrol, India.

*Corresponding authors E-mail: vasuthakkar@gmail.com.  Tel: +91-2692-234412. Fax: +91-2692-236475.

Accepted 22 July, 2006

Aim of the study was to examine the enhanced growth supporting conditions and production of jasmonic acid (JA) by Lasiodiplodia theobromae in liquid cultures. Seven different media were assessed for supporting the maximum growth of the L. theobromae. Basal salt media supported maximum growth on solid as well as liquid media. Optimum temperature and pH for growth of L. theobromae were found 30°C and 5.5, respectively. Besides studying influence of these environmental factors for growth, some other basic fermentation criteria for improvement of fermentation of JA using L. theobromae were standardized. This includes inoculum size and age, effect of aeration, effect of surface area and effect of sub-culturing the fungus.

Key words: Jasmonic acid, high performance thin layer chromatography, Lasiodiplodia theobromae, submer-ged fermentation.