African Journal of Biotechnology Vol. 6 (20), pp. 2314-2318, 18 October 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Antibacterial effects and toxigenesis of Penicillium aurantiogriseum and P. viridicatum

Mustapha Khaddor¹, Rabah Saidi¹, Anouar Aidoun¹, Ahmed Lamarti^2*, Abdelrhafour Tantaoui-Elaraki³, Mohammed Ezziyyani⁴, Maria-Emilia Candela Castillo⁴ and Alain Badoc⁵

¹École Normale Supérieure, BP 229, 93150 Martil, Morocco.

2Département de Biologie, Unité de Biotechnologie et d’Amélioration des plantes,Faculté des Sciences M’hannech II, BP 212, 93002 Tétouan, Morocco.

³Sup’Agro (École Supérieure de l’Agro-alimentaire), 22, rue le Câtelet, Belvédère, 20300, Casablanca, Morocco.

⁴Laboratorio de Fisiopatología, Departamento de Biología vegetal,

Facultad de Biología, Campus Universitario de Espinardo, 30100 Espinardo, Murcia, Spain.

⁵Laboratoire de Mycologie et Biotechnologie végétale, Faculté des Sciences Pharmaceutiques, Université Victor Segalen Bordeaux 2, 146, rue Léo Saignat, 33076 Bordeaux Cedex, France.

*Corresponding author. E-mail: lamarti.ahmed@menara.ma. Phone: (212) 39960569. Fax: (212) 39994500.

Abbreviations: ANIS: Solution of p-anisaldehyde in absolute ethanol - glacial acetic acid -concentrated sulfuric acid; CYA:  Czapeck Yeast extract Agar; ENS: École Normale Supérieure; MB: Malt Broth; MEA:  Malt Extract Agar; Nm:  Nanometre; RF:     Migration forehead; TLC: Thin Layer Chromatographie; TYG:  Tryptone Yeast extract Glucose; YES:  Yeast Extract Sucrose.

Accepted 25 January, 2007

The toxigenesis of one Penicillium aurantiogriseum and one Penicillium viridicatum isolates was investigated. Sterile culture filtrates of both fungi had a clear antibacterial effect only against Bacillus subtilis. The effect on B. subtilis varied with amount of filtrate used and temperature. The antibacterial activity of chloroform extracts varied with the nature of media used to grow the fungi. Different mycotoxins were identified in the fungal cultures using thin-layer chromatography. P. aurantiogriseum was found to produce penicillic acid, terrestric acid and aurantiamine, while penicillic acid, terrestric acid, brevianamide A and xanthomegnin were produced by P. viridicatum.

Key words: P. aurantiogriseum, P. viridicatum, mycotoxins, antibacterial activity.