African Journal of Biotechnology Vol. 6 (12), pp. 1378-1381, 18 June 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Rapid high performance liquid chromatographic determination of chlorpropamide in human plasma

M .T. Bakare Odunola^1*, I. S. Enemali¹, M. Garba¹ and O. O. Obodozie²

¹Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria.

²National Institute for Pharmaceutical Research and Development Idu, Abuja, Nigeria.

*Corresponding author. E-mail: mojitaibat@yahoo.com

Accepted 10 April, 2007

Samples were extracted with dichloromethane and the organic layer evaporated to dryness. The residue was dissolved in methanol, and 25 µl aliquot injected onto the column. Tolbutamide was used as the internal standard for chlorpropamide. The UV detector response was linear over the range 0 – 300 µg/ml, with a correlation coefficient of 0.999 and detection limit of 1.30 ng/ml. Within day and between-day assay variations was generally < 2.50%. No interference from endogenous constituent was observed. The utility of the method was demonstrated by determine chlorpropamide in samples from human volunteers following a single oral dose of 250 mg in drug interaction studies. The procedure is simple and fast, requiring small volumes of plasma.

Key words: Liquid chromatography, chlorpropamide, plasma, tolbutamide.