African Journal of Biotechnology Vol. 6 (14), pp. 1617-1622, 18 July 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Identification of micro satellite markers on chromosomes of bread wheat showing an association with karnal bunt resistance

Mukesh Kumar^1*, O. P. Luthra¹, N. R. Yadav², Lakshmi Chaudhary³, Navinder Saini², Rajiv Kumar⁴, Indu Sharma⁵ and V. Chawla¹

¹Department of Genetics, CCS Haryana Agricultural University, Hisar-125 004, India.

² Department of Biotechnology and Molecular Biology, CCS Haryana Agricultural University, Hisar-125 004, India.

³Department of Botany and Plant Physiology, CCS Haryana Agricultural University, Hisar-125 004, India.

⁴Department of Plant Breeding, GB Pant University of Agriculture and Technology, Pantnagar, India.

⁵Department of Plant Breeding, Punjab Agricultural University, Ludhiana, India.

*Corresponding author. E-mail: mukeshhau@yahoo.com. Tel: +91-9416409963 Fax: +91-0171-2822002.

Accepted 3 April, 2007

A set of 104 wheat recombinant inbred lines developed from a cross between parents resistant (HD 29) and susceptible (WH 542) to karnal bunt (caused by Neovossia indica) were screened and used to identify SSR markers linked with resistance to karnal bunt as these would allow indirect marker assisted selection of karnal bunt resistant genotypes. The two parents were analysed with 46 SSR primer pairs. Of these, 15 (32%) were found polymorphic between the two parental genotypes. Using these primer pairs, we carried out bulked segregate analysis on two bulked DNAs, one obtained by pooling DNA from 10 karnal bunt resistant recombinant inbred lines and the other similarly derived by pooling DNA from 10 karnal bunt susceptible recombinant inbred lines. Two molecular markers, Xgwm 337-1D and Xgwm 637-4A showed apparent linkage with resistance to karnal bunt. This was confirmed following selective genotyping of individual recombinant inbred lines included in the bulks. These markers may be useful in marker assisted selection for karnal bunt resistance in wheat.

Key words: Karnal bunt, Neovossia indica, Resistance, SSR and Wheat.