African Journal of Biotechnology Vol. 6 (14), pp. 1634-1638, 18 July 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Molecular characterization of some local and exotic Brassica juncea germplasm

Waqar Ali¹, Iqbal Munir¹, Mian Afaq Ahmad¹, Wisal Muhammad¹, Nisar Ahmed¹, Durrishahwar², Shahid Ali¹ and Zahoor A. Swati¹

¹Institute of Biotechnology and Genetic Engineering (IBGE) NWFP Agricultural University, Peshawar, Pakistan.

²Department of Plant Breeding and Genetics, NWFP Agricultural University Peshawar, Pakistan.

*Corresponding author. E-mail: iqmunir@hotmail.com, waqarali04@yahoo.com.

Accepted 14 June, 2007

The production of Brassica germplasm with a wider genetic base is essential for using them properly in the genetic improvement of rapeseed/mustard. During the present study, different RAPD (Random Amplified Polymorphic DNA) primers were used to estimate the genetic distances among thirty different genotypes in all possible combinations. Level of genetic polymorphism at DNA level observed during present study varied, but in general it was in the range of 21.54 to 59.36%. The dissimilarity coefficient matrix of these lines based on the data of four RAPD markers using UPGMA method was also used to construct a dendrogram. The dendrogram analysis indicated that the lines UCD-319/2, UCD-310/2 and P34/R3 were genetically apart from other lines that can be exploited in a synergistic way to create wider genetic base in local Brassica germplasm thus augmenting the Brassica breeding program.

Key words: Rapeseed, Brassica juncea, genetic diversity, RAPD, molecular markers.