African Journal of Biotechnology Vol. 6 (24), pp. 2776-2779, 17 December 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

Verifying the reliability of hybrid issued from the cross “Picholine marocaine clones X Picholine du Languedoc”

Jamal Charafi ^1,2, Bouchra Rahioui², Abdelatif El Meziane², Abdelmajid Moukhli³, Belkacem Boulouha³, Cherkaoui El Modafar² and Bouchaib Khadari^1*

¹UMR 1098 Développement et Amélioration des Plantes (DAP), INRA, 2 place Viala, 34 060 Montpellier cedex 1, France.

²Laboratoire de Biotechnologie de la Valorisation et Protection des Agro-Ressources. Faculté des Sciences et Techniques Guéliz, B.P. 549, 40 000 Marrakech, Morocco.

³UR Amélioration Génétique des Plantes, INRA, Marrakech, Morocco.

*Corresponding author. E-mail: khadari@supagro.inra.fr. Telephone: 33 4 99 61 21 49. Fax: 33 4 99 61 26 16.

Accepted 4 June, 2007

In order to verify the reliability of hybrid population issued from crossing between 3 clones of "Picholine marocaine" cultivar and the "Picholine du Languedoc" cultivar, the descendants and their parents were analysed using 35 microsatellite loci. No offspring resulted from self crossing of "Picholine marocaine" cultivar and 218 descendants among 220 analysed are legitimate. This study showed clearly a segregating population and may be used as a genetic material for linkage map construction and for phenotyping resistance traits related to Spilocaea oleagina disease.

Key words: Olea europaea, segregating population, microsatellite markers, QTL, Spilocaea oleagina.