African Journal of Biotechnology Vol. 6 (10), pp. 1221-1224, 16 May 2007   

ISSN 1684–5315 © 2007 Academic Journals        

Full Length Research Paper

PREX-1979: Modeling the first ever prototype of a new generation of microbicides for preventing HIV infection among high risk women

Wayengera Misaki

Restrizymes Corporation 4001 Steeles Av. West, North York, TO, ON, Canada M3N 2T8.E-mail: wmisaki@yahoo.com.

Accepted 7 May, 2007

Impregnating a proteolytic substrate of specifity to viral envelope with a restriction enzyme cleaving the HIV genome could generate a novel line of microbicide. Web Cutter version 2.0 aimed at identifying which enzyme can cleave HIV-1 and 2 genomes in 10 or > cuts was employed. Computational analysis indicate that of 291, 17 (5.8%) enzymes had 10 or more cuts in the HIV- 1/SIVcpz genome as compared to 25 (8.9%) for HIV-2/SIVsmm with 6 enzymes (Eco-130I, EcoT141, ApoI, AcsI, BssTII, and StyI) having a mutual ability to cleave HIV-1 and 2 in 10 and more cuts. Although not a proteolytic enzyme, the surfactant Savvy/C31G (Cellegy) was identified as a safe and close candidate for our proteolytic substrate. With biochemical compound modeling, it is possible to stably impregnate the center of surfactant/detergent molecule with a natural product. The PREX Model may be explored to develop novel lines of microbicides aimed at preventing HIV and other viral STI transmission in humans

Key words: HIV/AIDS, Novel Microbicides Strategies, Restriction Modification Systems, HIV genomes, Combination-Microbicides