African Journal of Biotechnology Vol. 5 (13), pp. 1245-1248, 3 July 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

In vitro regeneration of adult trees of Bambusa vulgaris

Aliou NDIAYE, Mamadou Saliou DIALLO, Dame NIANG and Yaye Kène GASSAMA-DIA

Laboratoire de Biotechnologies végétales, Département de Biologie Végétale Université Cheikh Anta DIOP de Dakar.

*Corresponding authors E-mail. ndiayealiou2000@yahoo.fr.

Abreviations: IAA, Indol-3-acetic acid ; IBA : Indol-3-butyric acid. NAA : α-naphthalene acetic acid. BAP : 6-benzylaminopurine. MS : Murashige and Skoog. MMS : modified Murashige and Skoog.

Accepted 31 May, 2006

We developed procedures for the regeneration of Bambusa vulgaris using nodal segments from adult plants. Optimal shoot growth was after 16 days cultivation on modified Murashige and Skoog (MMS) medium supplemented with 2 mg/l of BAP. Elongated shoots of B. vulgaris rooted (45.85%) when cultured in MMS + 20 mg/l IBA. The rooted and acclimated shoots were successfully transferred into the field with 100% of plantlets survival.

Key words: Bambusa vulgaris, micropropagation, adult.