African Journal of Biotechnology Vol. 5 (15), pp. 1370-1374, 3 August 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

Identification and differentiation of Ficus carica L. cultivars using inter simple sequence repeat markers

Ferdaous Guasmi, Ali Ferchichi*, Kadija farés, Leila Touil

Institut des Régions Arides - Medenine – Tunisia.

*Corresponding authors E-mail: ferchichi.ali@ira.rnrt.tn Tel: +216 75 633 846; Fax: +216 75 633 006.

Accepted 31 May, 2006

Information on germplasm diversity and relationships among elite materials is fundamentally important in crop improvement. The main objectives of our study is to determine the level of genetic diversity inter fig-tree cultivars using inter simple sequence repeat (ISSR) markers. Fifty-seven local Tunisian fig-tree cultivars were fingerprinted with ISSR marker. A total of 33 alleles were detected. A high level of genetic diversity was identified inter cultivars. The clustering grouped the studied cultivars into four clusters with no correlation to geographical origins.

Key words: Ficus carica L., genetic diversity, ISSR markers, Southeast Tunisia.