African Journal of Biotechnology Vol. 5 (8), pp. 629-634, 18 April 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

Assessment of the physiological and biochemical characterization of a Lactic acid bacterium isolated from chicken faeces in sahelian region

Ali Bayane^1,*, Dominique Roblain¹, Robin Dubois Dauphin¹, Jacqueline Destain¹, Brehima Diawara², Philippe Thonart¹

¹FUSAGx, Unité de Bio-industrie, Centre Wallon de Biologie Industrielle (CWBI). Passage des Déportés 2, 5030 Gembloux, Belgique.

²Département de Technologie Alimentaire, IRSAT/CNRST. 03 BP 7047 Ouagadougou 03 Burkina Faso.

*Corresponding author. E-mail: bioindus@fsagx.ac.be.

Accepted 12 December, 2005

Abstract

The aim of this work was the examination of the microbiological and technological properties of a lactic acid bacterium (CWBI-B623) isolated from chicken faeces in sahelian region (Burkina Faso). The strain CWBI-B623 is a Gram positive rod, asporogenous, catalase-positive, facultatively anaerobic, non motile and mesophilic. The analysis showed that it produce L(+)-lactic acid via homofermentative pathway and it was able to ferment an important number of the carbohydrates of API 50CHL system. The 16S-rDNA-sequence analysis revealed that the isolate was phylogenetically a member of the genus Lactobacillus and formed a subline within the Lactobacillus casei cluster. The minimal inhibitory concentration of CWBI-B623 for bile salts was higher than 1% and the survival rates to acidity with pH 3.0 and pH 2.5 were 26 and 1.2%, respectively. CWBI-B623 is an Lactobacillus casei species based on its physiological and biochemical properties and it could be a good candidate for probiotic formulations.

Key words: Lactic acid bacteria, Lactobacillus, probiotic, biodiversity.