African Journal of Biotechnology Vol. 5 (10), pp. 866-870, 16 May 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

Azide-based mutagenesis suppresses Striga hermonthica seed germination and parasitism on maize varieties

S. Kiruki*, L .A. Onek and M. Limo

Department of Biochemistry and Molecular Biology, Egerton University, P. O Box 536 Njoro, Kenya.

*Corresponding authors E-mail: kirusila@yahoo.com

Accepted 11 January, 2006

Abstract

Small-scale farmers currently produce more than 90% of the maize grown in Kenya.  However, the parasitic weed, Striga hermonthica has become a menace because it is a widespread parasitic weed causing severe damages to major cereals like maize and sorghum.  Mutant lines of maize were created in the laboratory using sodium azide (NaN₃) and their performance in respect to the degree of Striga resistance screened. The presence of active chemical stimulants in the root exudate was further investigated. This is the first report on mutagenic conversion of Striga-susceptible to Striga-resistant maize varieties (K9908, K9910 and K9911). Stable performances of the new varieties have been demonstrated in Striga-infested fields in western Kenya by agronomic researchers.

Key words: Azide-based mutagenesis, biochemical characterisation, Striga hermonthica.