African Journal of Biotechnology Vol. 5 (10), pp. 878-882, 16 May 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

Production, purification and partial characterization of lipase from Trichoderma Viride

M. Akram Kashmiri, Ahmad Adnan* and Beenish Waseem Butt

Department of Chemistry, G C University, Lahore, Pakistan

*Corresponding authors Email:adnan_biochem@yahoo.com. Tel: 0092-42-111 000 010 ext. 266.

Abbreviations: V_max, Maximum velocity; K_m, Michaelis Constant; Y_X/S, Growth yield; m, Specific growth rate; U, Lipase unit; LSD, Least significant difference; DEAE, Diethyl amino ethyl.

Accepted 14 February, 2006

Abstract

A new strain of lipolytic Trichoderma viride was isolated from the soil on a selective medium that contained olive oil as the only source of carbon and energy. The isolated strain was cultivated for lipase production in shake flasks at 30±1^oC and the fermentation pattern was studied. The maximum extracellular lipase activity of 7.3 U/mL and the maximum intracellular activity of 320 U/g mycelium were noted after 48 h. Although maximum fungal biomass was present at 13.6 g/L at 60 h but highest specific growth rate was observed between 6 and 18 h. The extracellular lipase present in the broth was purified 134-folds with an overall yield of 46% through purification procedure of ammonium sulphate precipitation, ion exchange and gel permeation chromatography. The K_m value of the purified enzyme for triolein hydrolysis was found to be 1.229 m.mole/L.

Key words: Fermentation, specific growth rate, fungal lipases, Michaelis constant, yield.