African Journal of Biotechnology
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African Journal of Biotechnology Vol. 5 (6), pp. 490-496, 15 March 2006 ISSN 1684–5315 © 2006 Academic Journals
Cloning and characterization of maize
ZmSPK1, a homologue to nonfermenting1-related protein kinase2 Huawen Zou1,2F,
Xiuhai Zhang2F, JiuRan
Zhao3, Qing Yang1, Zhongyi Wu2, Fengge
Wang3 and Conglin Huang2* 1College
of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2Beijing
Agro-Biotechnology Research Center, Beijing Academy of Agriculture and
Forestry Sciences, Beijing 100089, China. 3Maize
Research Center, Beijing Academy of Agriculture and Forestry Sciences,
Beijing 100089, China.
*Corresponding authors
E-mail: haungconglin@hotmail.com.
Accepted 23
January, 2006 |
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| Abstract | |||||
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SnRK2s play important roles in plant stresses responses. One full-length cDNA encoding a SnRK2b homologue was isolated from maize by RT-PCR and named as ZmSPK1 (for stress-induced protein kinase). The ZmSPK1 protein has 364 amino acids with an estimated molecular mass of 41.8 KD and an isoelectric point of 5.8. The deduced protein sequence has the closest identities to the members of SnRK2b group. RT-PCR analysis showed that the ZmSPK1 expression was induced by mannitol, salt and abscisic acid (ABA). Furthermore, in different tissues the ZmSPK1 showed different expression patterns and was most abundant in reproductive organs. These results suggested that ZmSPK1 might play multiple roles in abiotic stress resistance pathways, as well as in plant reproductive development.
Key words: Zea mays L., SnRK2b, expression pattern, abiotic stress. |
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