African Journal of Biotechnology Vol. 5 (6), pp. 497-502, 15 March 2006   

ISSN 1684–5315 © 2006 Academic Journals        

Full Length Research Paper

Molecular characterization of Fusarium oxysporum f. sp. ciceri causing wilt of chickpea

Bhim Pratap Singh¹*, Ratul Saikia², Mukesh Yadav¹, Rakesh Singh¹, V.S.Chauhan³ and Dilip K. Arora¹

¹National Bureau of Agriculturally Important Microorganisms, Kushmaur, Mau 275101, India.

²Division of Biotechnology, Regional Research Laboratory (CSIR), Jorhat 785006, India

³Department of Biotechnology, Bundelkhand University, Jhansi, India.

*Corresponding author E-mail: bheem_50@yahoo.co.in, ratulsaikia@yahoo.com.

Accepted 16 February, 2006

Thirty isolates of Fusarium oxysporum f. sp. ciceri were isolated from rhizosphere soil of chickpea from different locations in Northern India. The amount of genetic variation was evaluated by polymerase chain reaction (PCR) amplification with a set of 40 RAPD primers and 2 IGS primers. Less than 10% of the amplified fragments in each case were polymorphic. Genetic similarity between each of the isolates was calculated and results indicate that there was little genetic variability among the isolates collected from the different locations. At the 0.75 similarity index the isolates divides into three groups. Isolates Foc-A18, Foc-A19, Foc-A20 forming a similar group and far different from other isolates.

Key words: Fusarium oxysporum f. sp. ciceri, Fusarium wilt of chickpea, RAPD, ITS, IGS.