Utilization of carbon and nitrogen sources by Streptomyces  kanamyceticus M 27 for the production of an Anti bacterial antibiotic

ANURAG PANDEY¹*, ANUPAM SHUKLA¹   and S.K. MAJUMDAR²

¹Department of Biotechnology And Bio-Medical Engineering, Government, Engineering College, Raipur - 492010, Chhattisgarh State – India.

²Department of Life Science & Biotechnology, Jadavpur University, Kolkata- India-32.

*Corresponding Author E-Mail: anurag2005@rediffmail.com; Tel: +91-094252-44348.

Accepted 14 June, 2005

We tested a number of carbon and nitrogen compounds for their effect on the production of an antibacterial antibiotic by Streptomyces kananmyceticus M27. Dextrose was found to be the most suitable carbon source, though maltose, sucrose, and soluble starch gave moderate yields. (NH₄)H₂PO₄ and yeast extract were adequate nitrogen sources for antibiotic production. There was, however, no direct relation between the growth of the organism and antibiotic formation. The pH of the medium might be an important factor for antibiotic formation, as media giving high antibiotic yields showed an alkaline pH.

Key words: Nitrogen compounds, Streptomyces kananmyceticus M27, dextrose, antibacterial antibiotic