African Journal of Biotechnology Vol. 4 (10), pp. 1180-1184, October 2005          
ISSN 1684–5315 © 2005 Academic Journals

Full Length Research Paper

Cloning and expression of Phanerochaete chrysosporium cellobiohydrolase (cbhI.2)

Howard RL

Microbiology, School of Molecular and Life Sciences, University of Limpopo, P/Bag X1106, Sovenga, 0727, South Africa.  E-mail: howardr@ul.ac.za. Tel/fax:+27 15 268 2862.

Accepted 27 September, 2005

The Phanerochaete chrysosporium ME446 cbhI.2 cDNA was cloned and expressed in Escherichia coli using a pET system. Pulse-labelling revealed the expression of an induced protein of approximately 50-60 kDa that reacted with anti-P. chrysosporium ME446 CBH antibodies. The expressed protein remained undegraded in vivo for 135 min but was biologically inactive in vitro.     

Key words: Phanerochaete chrysosporium, cbhI.2, cloning and expression, pulse-labelling.