African Journal of Biotechnology
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African Journal of Biotechnology Vol. 4 (3), pp. 247-250, March 2005
Quantitative trait loci for head-bug
resistance in sorghum
M. Deu1, A. Ratnadass2*, M. A. Hamada2,
J.L. Noyer3, M. Diabate2† & J. Chantereau4
1CIRAD-CA, UMR 1096/PIA, TA 40/03, F-34398 Montpellier, France
2ICRISAT-CIRAD, BP 320 Bamako, Mali
3CIRAD-AMIS, UMR 1096/PIA, TA 40/03, F-34398 Montpellier,
France
4CIRAD-CA, Programme CALIM, TA 73/09, F-34398 Montpellier,
France
Corresponding author. E-mail:
ratnadass@cirad.fr; Tel: +261 20
44 49236.
Accepted 23
December, 2004
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| Abstract | |||||
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QTLs were mapped in F2 progeny derived from a cross between the head-bug resistant sorghum cultivar Malisor 84-7 and susceptible S 34. The phenotypic evaluation was conducted in Mali. The mapped population consisted of 217 F2 plants, with 345 homologous and heterologous RFLP probes and 49 microsatellite markers tested. Eighty-one RFLP markers revealed polymorphism between the two parents, and 14 microsatellite markers gave usable amplification products. A genetic map including 92 loci distributed over 13 linkage groups, and covering a total distance of 1160 cM was built. Three significant and seven putative QTLs were detected and placed on the map.
Key words: Head-bug, Eurystylus oldi, sorghum, resistance, RFLP, microsatellite, QTL.
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