African Journal of Biotechnology Vol. 4 (1), pp. 45-49, January 2005   

ISSN 1684–5315 © 2004 Academic Journals        

Full Length Research Paper

Study on transformation of cowpea trypsin inhibitor gene into cauliflower (Brassica oleracea L. var. botrytis)

Lv Lingling¹*   Lei Jianjun²   Song Ming³    Li Liyun⁴   Cao Bihao⁵

¹Southern Subtropical Crop Research Institute,  Zhan Jiang, Guang Dong , 524091;

²South China Agricultural University, Guang Zhou, Guang Dong , 510642;

³Southwest Agricultural University, Chong Qing, 400716;

⁴Agriculture & Industry & Commerce Secondary professional School, Zhan Jiang, Guang Dong , 524091 )

*Corresponding author. E-mail: lianzi9381@163.com.  

Accepted 26 August, 2004

Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower by agrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary insect-resistant assay showed that the transgenic plants were more resistant to Pieris rapae than non-transgenic plants.

Key words: cauliflower (Brassica oleracea L. var. botrytis)   agrobacterium -mediated   genetic transformation     CpTI gene    transgenic plant.