African Journal of Biotechnology Vol. 4 (1), pp.14-18, January 2005  

ISSN 1684–5315 © 2004 Academic Journals      

Full Length Research Paper

Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils

Bertrand Tatsinkou Fossi¹, Frederic Tavea¹, Robert Ndjouenkeu^1*

Department of Food Science and Nutrition, University of Ngaoundéré, P.O.Box 455 Ngaoundéré, Cameroon.

*Corresponding author. E-mail: rndjouenkeu@yahoo.fr.

Accepted 14 July, 2004

An amylolytic yeast strain was isolated from starchy soils (flour mills environment, flour market, cassava farms after harvesting and transformation of tubers) and its enzyme productivity and activity evaluated. The enzyme synthesis was optimum at 30°C when initial pH of fermentation medium was 4.5. After extraction and partial purification, the enzyme remained stable in a range of temperature and pH between 20°C-60°C and 2-8 pH respectively. The optimum enzyme activity was displayed at 70°C and pH 5.5 and 6.5. The thermostability of the enzyme was comparable to that of amylases from bacteria.

Key word: Amylolytic yeast, amylase activity, thermostable amylase.