African Journal of Biotechnology Vol. 4 (2), pp. 151-156, February 2005          
ISSN 1684–5315 © 2005 Academic Journals

Full Length Research Paper

Isolation and characterization of micro satellites in Bambusa arundinacea and cross species amplification in other bamboos

SUMITRA NAYAK and GYANA RANJAN ROUT *

Plant Biotechnology Division, Regional Plant Resource Center, Nayapalli, Bhubaneswar –751 015, Orissa, India

*Corresponding author.  E-mail: grrout@hotmail.com.

Accepted January 15, 2005

Isolation and characterization of microsatellites was analyzed in Bambusa arundinacea and cross species amplification in other bamboos. Six microsatellites, three polymorphic and three monomorphic, were characterized in a bamboo species, Bambusa aruninacea belonging to the family Poaceae. The numbers of alleles per locus ranges form 2 to 6. Allelic diversity ranges from 0.128 to 0.789. Polymorphic Information Content (PIC) values for two loci were > 0.3, as an indicator of polymorphic allele. Cross species amplification has been tested in other 18 bamboo species. Monomorphic simple sequence repeats (SSRs) have been found to be cross amplified in most of the tested species while polymorphic ones in only three to four species. Utilization of the SSR loci in genetic diversity study of B. arundinacea and other cross amplified bamboo species is discussed.

Key words: Bambusa arundinacea, microsatellite marker, cross species amplification, population study, species identification.