African Journal of Biotechnology Vol. 4 (12), pp. 1378-1383, December 2005          
ISSN 1684–5315 © 2005 Academic Journals

Full Length Research Paper

Agrobacterium-mediated transformation of plantain (Musa spp.) cultivar Agbagba

L. Tripathi*, J. N. Tripathi and J. d'A. Hughes^Y

International Institute of Tropical Agriculture (IITA), PO Box 7878, Kampala, Uganda

^YCurrent Address- AVRDC-The World Vegetable Center, P.O. Box 42, Shanhua, Tainan 74199, Taiwan.

*Corresponding authors E-mail: l.tripathi@cgiar.org, Tel: 256-75-787817, Fax: 256-41-223494.

^YCurrent Address- AVRDC-The World Vegetable Center, P.O. Box 42, Shanhua, Tainan 74199, Taiwan.

Accepted 4 October, 2005

An Agrobacterium-mediated plant transformation system was developed for the production of transgenic plantain [Musa spp. cultivar Agbagba (AAB)]. Apical shoot tips were transformed using Agrobacterium strain EHA105 with the binary vector pCAMBIA 1201, having the hygromycin resistance gene as a selection marker and GUS-INT as a reporter gene. Transient expression of the b-glucuronidase (uid A) gene was achieved in transformed apical shoot tips. The hygromycin resistant shoots were regenerated 4 to 5 weeks after co-cultivation of explants with Agrobacterium. The two step selection procedure allowed the regeneration of shoots which were uniformly transformed. The integration of the uid A gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. In this study, transformation based on regeneration from apical shoot tips has been demonstrated. This process does not incorporate steps using disorganized cell cultures but uses micropropagation, which has the important advantage that it allows regeneration of homogeneous populations of plants in a short period of time. This study shows the enormous potential for genetic manipulation of Musa species for disease and pest resistance, as well as abiotic factors, using a rapid and non-species specific transformation and regeneration system.

Key words: Plantain, Musa, Agrobacterium-mediated transformation, apical shoot tip.