African Journal of Biotechnology Vol. 4 (12), pp. 1404-1408, December 2005          
ISSN 1684–5315 © 2005 Academic Journals

Full Length Research Paper

Inhibition of aflatoxin-producing aspergilli by lactic acid bacteria isolates from indigenously fermented cereal gruels

A. A. Onilude*, O. E. Fagade, M. M. Bello and I. F. Fadahunsi

Microbial Physiology and Biotechnology Research, Department of Botany and Microbiology, University of Ibadan, Ibadan, NIGERIA.

*Corresponding author. E-mail: onealbee2000@yahoo.com, Fax: 234 - 02 – 8103118.

Accepted 22 August, 2005

A total of six lactic acid bacteria (LAB) isolates were selected from five indigenously fermented cereal gruels and identified as Lactobacillus fermentum OYB, Lb. fermentum RS2, Lb. plantarum MW, Lb. plantarum YO, Lb. brevis WS3, and Lactococcus spp. RS3. Six aflatoxin-producing aspergilli were also selected from the various food sources. Two of the isolates, identified as Aspergillus parasiticus C2 and A. parasiticus AF7, produced both aflatoxin B₁ and G while the other four identified as A. flavus M1, A. flavus B4, A flavus B5 and A. flavus C6, produced only aflatoxin B₁. Each of the LAB isolates inhibited the growth of at least a toxin-producing Aspergillus. The maximum inhibitions were shown by Lb. plantarum YO, which was able to inhibit the vegetative and sporulative growth of all the aflatoxin-producing aspergilli. Lactococcus spp. RS3 and Lb. brevis WS3 were only able to inhibit A. parasiticus C7 reasonably and A. flavus B5 and C6 mildly.

Key words: Inhibition, Lactic acid bacteria, aflatoxin-producing aspergilli.