African Journal of Biotechnology
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African Journal of Biotechnology Vol. 4 (8), pp. 844-846, August 2005
Production of a
new anti-A monoclonal reagent
Fouad Sadiq1,2*, Ahmed Tissent1,2,
Norddine Habti1, Driss Radallah2, Najat El Amrani2,
Noufissa Benchemsi1 1Laboratoire de Génie Génétique et
Cellulaire. Service d’Hématologie. Faculté de Médecine et de Pharmacie.
Casablanca, MAROC. 2Département de Biologie. Faculté des
Sciences Ben M’sik. Casablanca, MAROC. Accepted 8 March, 2005
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| Abstract | |||||
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Monoclonal antibodies are essential tools in molecular and cellular immunology research. They have essentially replaced the polyclonal antibodies in identifying blood groups and detecting cell markers and pathogenic agents. The aim of the present study is to produce monoclonal antibody identifying the ABO blood groups using the murine hybridoma technology. An anti-A monoclonal antibody A907 was selected and estimated for its use in the manufacture of a reagent anti-A. The selected antibody specifically reacts with A1, A2, A1B and A2B erythrocytes. It does not recognize B, O, A3 and Ax erythrocytes. The A907 monoclonal antibody can be used in blood grouping in association with a reagent recognizing the A weak phenotypes.
Key words: Anti-A monoclonal antibodies, murine myeloma cells, haemagglutination. |
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