African Journal of Biotechnology Vol. 4 (4), pp. 326-331, April 2005          
ISSN 1684–5315 © 2005 Academic Journals

Full Length Research Paper

The effect of media, oil type and rate on the mycelia wet and dry weights of Lentinus squarrosulus (Mont.) singer and psathyrella patroumbonata pegler in submerged liquid culture

NWANZE pi^1*, KHAN AU², AMEH JB³ and UMOH VJ⁴

¹Department of Biological Sciences,College of Natural and Applied Sciences, Igbinedion University,Okada, P.M.B. 0006,Edo State, Nigeria.

²Department Of Biological Sciences, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.

³Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.

⁴Department Of Microbiology, Ahmadu Bello University, Zaria. Kaduna State, Nigeria.

*Corresponding author.  E-mail: stonenwanze@yahoo.com; Tel: 2348053067394

Accepted 28 February, 2005

Lentinus squarrosulus and Psathyrella atroumbonata, two edible indigenous mushroom species, were cultured in four different submerged liquid media supplemented with coconut, cotton, groundnut, butterfat, palm kernel and palm oil at 5 different rates. The differences in the means due to the different media types were highly significant (p<0.01) for the mean mycelia wet and dry weights of both L. squarrosulus and P. atroumbonata. SLCM3, which contained twice the glucose level present in each of the other three liquid culture media, induced the heaviest mean wet and dry mycelia weights of both mushroom species. Oil type had a highly significant (p<0.01) effect on the wet and dry mycelia weights of L. squarrosulus as well as the mycelia wet weight of P. atroumbonata. Butterfat induced the heaviest weights. In addition, oil rate also had a significant effect (p<0.01) on the mean mycelia wet and dry weights of both mushroom species. The oil rates of 0.003, 0.005 and 0.007 ml/ml produced comparable optimum mean mycelia wet and dry weights of the mushrooms.

Key words: Lentinus squarrosulus, Psathyrella atroumbonata, supplemented media, submerged liquid media, oil type and rate.