African Journal of Biotechnology Vol. 3 (9), pp. 477-480, September 2004           
ISSN 1684–5315 © 2004 Academic Journals

Full Length Research Paper

Prevalence of bla_SHV  genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of bla_SHV11-like gene

Boukaré Zeba¹* Pere Jacques Simporé², Odile G. Nacoulma¹, Jean-Marie Frere³

¹Laboratoire de Biochimie et de Chimie appliquée/ Enzymologie. UFR-SVT/ Université de Ouagadougou/ Burkina Faso. ²Saint Camille medical center/ Ouagadougou/ Burkina Faso.

³Enzymologie/ Centre d’ingenierie des protéines / Ulg/ Belgique.

*Corresponding author. E-mail: b_zeba@yahoo.fr. Fax: (226) 33 06 08. Tel: (226)29 08 33.

Accepted 13 August, 2004

Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to b-lactam antibiotics are investigated to isolate the genes responsible of b-lactamase activity. The use of engineering techniques enables us to show the widespread of bla_SHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical bla_SHV-11  gene.

Keys words: b-Lactamases, Klebsiella  pneuminiae,  bla_SHV  gene, Saint  Camille medical centre, Ouagadougou.