African Journal of Biotechnology Vol. 3 (6), pp. 334-338, June 2004
ISSN 1684–5315 © 2004 Academic Journals

Full Length Research Paper

Antimicrobial resistance of bacterial strains isolated from orange juice products

LATEEF, A.*, OLOKE, J.K., AND GUEGUIM-KANA, E.B.

Department of Pure and Applied Biology, Ladoke Akintola University of Technology, PMB 4000, Ogbomoso, Nigeria.

*Corresponding author. Tel.: +234-0803-740-0520. E-mail: agbaje72@yahoo.com.

Accepted 11 June 2004

Forty samples of twenty brands of sachet orange juice products were examined microbiologically. All the products were contaminated with bacteria and yeasts. The organisms encountered include Saccharomyces cerevisiae, Saccharomyces sp, Rhodotorula sp, Bacillus cereus, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes and Micrococcus sp. The resistances of thirty bacterial strains isolated from orange juice products to the commonly used antibiotics were studied. About 66.67% of the isolates were resistant to augmentin and amoxycillin; 63.33% to cotrimoxazole, 56% to cloxacillin, and 23.33% to tetracycline. Resistances of 10, 6.67, and 3.33% were obtained for gentamicin, erythromycin and chloramphenicol respectively. Among the eight antibiotics tested, seven patterns of drug resistance were obtained. Six out of these are multiple-drug resistance with number of antibiotics ranging between 2 to 8. While MIC of amoxycillin ranged between 10-25mg/ml for the strains of E. coli, MIC of 10-20mg/ml was obtained for the strains of S. aureus. The MIC for cloxacillin was 0.1-1.0mg/ml for E. coli strains, and 0.01-1.0mg/ml for S. aureus strains. In all, ten strains of the bacterial isolates had evidence for the production of β-lactamases.

Keywords: Orange juice, antibiotics, resistance pattern, b-lactamase, microbiological standard.