African Journal of Biotechnology
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African Journal of Biotechnology Vol. 3 (1), pp. 63-66, January 2004 ISSN 1684–5315 © 2004 Academic Journals
Effect of carbon and nitrogen sources and incubation times on poly-beta-hydroxybutyrate (PHB) synthesis by Bacillus subtilis 25 and Bacillus megaterium 12
Z. Nur Yüksekdağ1*, Belma Aslım1, Yavuz Beyatlı1, Nazime Mercan2
1Department of Biology, Faculty of Science and Arts, Gazi University, Teknikokullar, Ankara-TURKEY 2Department of Biology, Faculty of Science and Arts, Pamukkale University, Denizli-TURKEY
*Corresponding Author. E-mail: zehranur@gazi.edu.tr. Tel: +09-312-2126030/2702. Fax: +09 2122279.
Accepted 3 December 2003 |
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| Abstract | |||||
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In this study, poly-beta-hydroxybutyrate (PHB) production by the Bacillus subtilis 25 and Bacillus megaterium 12 strains was investigated in nutrient broth medium at different incubation times (between 6 h and 48h). The best PHB production and all yields of these strains were determined. The productions were 0.101 g/L, 0.142 g/L and the percentage yields were 18.03%, 14.79% after 45h, respectively. At 48th h, there was a decrease in PHB yields. In our study, the effects of different carbon and nitrogen sources on PHB production in these strains were also tested. While the strains produced less PHB in nutrient broth medium with different carbon and nitrogen sources, the highest level of PHB accumulation of the strains was observed in the medium with protease peptone. In this nutrient broth medium with protease peptone the percentage PHB yield of B. subtilis 25 was determined as 78.69%, while in the same nitrogen sources this percentage in B. megaterium 12 was determined to be 77.00%.
Key words: Poly-b-hydroxybutyrate, Bacillus subtilis, B. megaterium , Different carbon and nitrogen sources, incubation time.
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